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KMID : 0043320090320101385
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Archives of Pharmacal Research
2009 Volume.32 No. 10 p.1385 ~ p.1392
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Cimiside E Arrests Cell Cycle and Induces Cell Apoptosis in Gastric Cancer Cells
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Kim Yeong-Shik
Guo Lian-Yu
Joo Eun-Ji
Son Kun-Ho
Jeon Su-Jin
Jang Se-Hyun
Shin Eun-Myoung
Zhou Hong-Yu
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Abstract
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Cimiside E was isolated from the Cimicifuga heracleifolia Komarov extract, which has been previously demonstrated to possess apoptotic action on gastric cancer cells. The IC50 value of cimiside E on gastric cancer cells for 24 h was 14.58 μM. The mechanism of apoptosis was further elucidated through western blot, RT-PCR, morphology, Annexin V-FITC/PI staining and cell cycle analysis. Cell cycle arrest was induced by cimiside E in S phase at a lower concentration (30 μM) and G2/M phase at higher concentrations (60 and 90 μM). Cimiside E mediated apoptosis through the induction of the caspase cascade for both the extrinsic and intrinsic pathways. These findings suggest that cimiside E may be an effective chemopreventive agent against cancer.
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KEYWORD
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Cimicifuga heracleifolia Komarov, Cimiside E, Apoptosis, Gastric cancer cells
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